Detection of Novel Mutation in LukS Panton-Valentine Leukocidin Gene in Twelve Isolates of Staphylococcus aureus from Sudanese Patients

Document Type : Research Article

Authors

• Najem Aldin Mohammed Osman ¹
• Intisar Elhag Elrayah ²
• Hisham Altayb ³
• Nihad Mohammed Elhaj ⁴
• Mohamed Ahmed Salih ⁵
• Nadir Abuzeid ⁶
• Muataz Mohmed Eldirdery ⁴
• Yassir Mahgoub Mohamed ⁴

¹ Department of Biotechnology, Faculty of Science and Technology, Omdurman Islamic University, Sudan

² College of Applied Medical Science, Shaqra University, KSA

³ Department of Microbiology, College of Medical Laboratory Sciences, Sudan University for Science and Technology

⁴ Department of Microbiology, Tropical Medicine Research Institute, National Center for Research, Sudan

⁵ Department of Biotechnology, Biotechnology Park, Africa City of Technology, Sudan

⁶ Faculty of Medical Laboratory Sciences, Omdurman Islamic University

Abstract

Staphylococcus aureus carrying PVL gene remain major health problem associated with highly virulent infections. Characterization of such gene is important to know the impact and the functional significance of nucleotide variations. PCR and standard sequencing were performed for twelve Sudanese strains from different sources. Protein structures prediction, modeling and physiochemical analysis were done to analyze genetic variation in PVL. Single nucleotide polymorphism at position 610 (A / G) of lukS-PV was detected in all tested isolates resulted in nonsynonymous mutation in the amino acid sequence at position 204 (N / D). Using molecular and bioinformatics tools the detected novel mutation in lukS-PV may have a diagnostic functional significance.

Keywords

• S. aureus
• PVL
• lukS-PV
• Mutation
• Sudanese

Main Subjects

• Molecular Microbiology

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