Detection of Human Papillomavirus in Osophageal Squamous Cell Carcinoma samples in Mazandaran Province, Iran

Document Type : Research Article

Authors

• Mahtab Alijanpour Aghamolkei ¹
• Alijan Tabarraei ²
• Abdolvahab Moradi ¹
• Farzin sadeghi ³
• Yousef Yahyapour ⁴

¹ Department of Medical Virology, Golestan University of Medical Sciences, Gorgan, Iran

² infectious diseases research center, Golestan University of Medical Sciences, Gorgan, Iran

³ Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

⁴ Infectious Diseases & Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran

Abstract

We examined 170 formalin-fixed paraffin-embedded samples from esophageal squamous cell carcinoma patients. All subjects live in Mazandaran province, a region with high incidence rate of esophageal cancer and have become known as the “Asian Esophageal Cancer Belt”. Samples were tested for HPV-DNA by MY09/11 and Gp5+/6+ general primers using nested PCR. Of the 170 ESCC samples, 86 (50.6%) were male and 84 (49.4%) were female. The mean age of the subjects was 66.5±11.1 and ranged from 35 to 91 years. Totally, HPV-DNA was detected in 62 (36.5%) of the esophageal squamous cell carcinoma samples by HPV L1 consensus primers. Considering the location of esophagus specimens, of 62 positive samples, 16 (25.8%) samples were in the upper third, 28 (45.2%) in the middle third, and 18(29.0%) in the lower third.  The current study showed a relatively substantial prevalence of HPV infection in esophageal squamous cell carcinoma samples in Mazandaran province.

Keywords

• HPV
• Nested PCR
• Osophageal Cancer
• Mazandaran
• Iran

Main Subjects

• Virology

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