Isolation and Identification of extremophiles producing anticancer L-glutaminase and L- Methioninas enzymes from Yale Gonbad hot spring in Qazvin, Iran
1
Department of biology, East Tehran branch, Islamic Azad University, Tehran, Iran
2
Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
Abstract
L Glutaminase and L- methioninase, therapeutic enzyme obtained from microbial source have been considered in the treatment of cancer. A total 20 water samples were obtained from the Yale Gonbad hot spring in Qazvin province, Iran. Production capabilities L Glutaminase and L- methioninase were tested on m9 and mcd plates, respectively. Enzyme-producing isolates were identified by conventional test and then confirmed using molecular methods. The PCR amplicons were directed for sequencing and Phylogenetic tree were drawn using Mega version 6 software. Twelve colonies were obtained from all tested samples. Three extratermophilic isolates showed L-glutaminase activity in M9 medium but only 1 isolates have L-methioninase activity in MCD medium. The phylogenetic tree drawn from the 16 S rRNA gene sequence showed were 100% similar to B. licheniformis and strains of S. epidermidis and L. sphaericus. In this study, Bacillus licheniformis was identified as a dominant and a native strain for the production of L-glutaminase and L-methioninase.
Farahmand, M., Azizi, E., & Shahhosseini, M. H. (2018). Isolation and Identification of extremophiles producing anticancer L-glutaminase and L- Methioninas enzymes from Yale Gonbad hot spring in Qazvin, Iran. International Journal of Molecular and Clinical Microbiology, 8(2), 1032-1038.
MLA
Mahnaz Farahmand; Elham Azizi; Mohammad Hassan Shahhosseini. "Isolation and Identification of extremophiles producing anticancer L-glutaminase and L- Methioninas enzymes from Yale Gonbad hot spring in Qazvin, Iran". International Journal of Molecular and Clinical Microbiology, 8, 2, 2018, 1032-1038.
HARVARD
Farahmand, M., Azizi, E., Shahhosseini, M. H. (2018). 'Isolation and Identification of extremophiles producing anticancer L-glutaminase and L- Methioninas enzymes from Yale Gonbad hot spring in Qazvin, Iran', International Journal of Molecular and Clinical Microbiology, 8(2), pp. 1032-1038.
VANCOUVER
Farahmand, M., Azizi, E., Shahhosseini, M. H. Isolation and Identification of extremophiles producing anticancer L-glutaminase and L- Methioninas enzymes from Yale Gonbad hot spring in Qazvin, Iran. International Journal of Molecular and Clinical Microbiology, 2018; 8(2): 1032-1038.
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