The study of Cumin oil essence on the function of FUM1 genes in Fusarium verticillioides by molecular analysis

Document Type : Research Article

Authors

• Alireza mokhtari ¹
• saeed hosseini ²
• shahnaz pourhossein ³
• nahid soleimanifard ⁴

¹ department of microbiology, faculty of medicine, Tehran university of medical science.

² Fardis Laboratory Complex, Food and Drug Laboratory, Fardis, Iran.

³ Biochemistry, Fardis Laboratory Complex, Food and Drug Laboratory, Fardis, Iran.

⁴ Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran.

Abstract

Fusarium is one of the most important pathogens of cereals; the fungal infection has increased in recent years due to ecological changes and soil nitrogen content increase. In the current study the effect of cumin oil essence on fusarium verticillioides by molecular method was investigated. The study included 14 isolates, 11 isolates of fusarium verticillioides and 3 Fusarium non verticillioides samples (negative control) were tested. For testing MIC and MFC, the RPMI1640 culture in microplate was used. After a week, the cultured RNA samples using the RNA extraction kit of Fermentas company (Purification Mini Kit GeneJET Plant RNA) was extracted from fungal biomass and the RT-PCR test along with the FUM1 gene was performed. The RT-PCR analysis displayed that FUM1 gene was expressed in fungal samples that were not under the effects of oil essence, but the gene was not expressed in samples after the cumin impact and there was not any band in agarose gel. the B1 fumonisin toxin which was produced by the fungus fusarium verticillioides contains several genes. The gene FUM1was involved toxin gene synthesis pathway of this toxin as the most essential gene.

Keywords

• Fusarium verticillioides
• Fumonisin
• cumin
• FUM1
• ITS

Main Subjects

• Antimicrobial agents

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