An Investigation on the Toxic Effect of Acetamiprid on Blood Factors, Protein Total, Lactobacillus in the Intestine of Cyprinus Carpio

Document Type : Research Article

Authors

• Zahra dorostgou ¹
• Zeynab Dorostguo ²
• fatereh rezaei ¹
• Azam Ghorbannia Delavar ³
• Saeed Alinejad Moallem ¹

¹ Department of Biology and Laboratory Science, Babol Branch, Islamic Azad University, Babol, Iran.

² Group of Biology, Kavian Institute of Higher Education, Mashhad, Iran.

³ Department of Biology, Payame Noor University (PNU), Post Bax – 19395 – 3697, Tehran, Iran.

Abstract

Many pesticides enter aquatic ecosystem after use and act as environmental pollutants. Changes in the biochemical parameters of fish blood serum can be considered as a suitable factor for detecting the effects of toxicity and determining the physiological status of fish exposed to pesticides. Lactic acid bacteria are also the most common types of bacteria present in the digestive tract of fish which provide substantial advantages for them. The objective of this research was to investigate the influence of Acetamiprid pesticide on blood factors, protein total and lactobacilli in the intestines of the Cyprinus Carpio. For this purpose, Cyprinus Carpio were exposed to 17, 34, 56, 85 and 113 micrograms per liter of Acetamiprid pesticide for 35 days and its effect on protein, red blood cell, hemoglobin, hematocrit, white blood cell and lymphocyte were studied. The results of biochemical studies revealed that after 35 days, the levels of red blood cells, hemoglobin, hematocrit, white blood cells, and lymphocytes in fish blood significantly decreased. However, there was a remarkable increase in the total blood protein compared to the control sample. Also, after separating the G + bacilli from the intestine of the fish and examining its anti biogram with different doses of Acetamiprid, the growth of lactose bacilli was not observed in all doses. Due to the effect of Acetamiprid on blood biochemical parameters and intestinal bacilli, it seems that the use of this pesticide can detrimentally affect the survival of Cyprinus Carpio.

Keywords

• red blood cells
• hemoglobin
• hematocrit
• white blood cells
• insecticides

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