The role of Herpes Simplex Viruses in triggering type 2 diabetes

Document Type : Research Article

Authors

• Sanaz Vahabi
• Atousa Ferdousi
• Mohammad Hassan Shahhosseini

Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran

10.22034/ijmcm.2022.1963794.1250

Abstract

Recently, some common viruses, like Herpes Simplex Virus ‎‎(HSV), have been ‎‎‎proposed as a risk factor to develop type 2 diabetes. However, still there is not ‎‎‎enough literature to ‎determine its importance as a triggering or risk factor. This ‎‎‎case-control study is designed to ‎evaluate the Attendance rate of the HSV genome ‎in ‎‎serum samples of diabetic ‎patients.‎ The study and control groups included ‎‏50‏‎ ‎serum samples of diabetic type 2 and 50 non-diabetic respectively. The detecting ‎PCR test for HSV genome types 1 and 2 with common DNA polymerase gene ‎region, as the target gene, was optimized. Demographic parameters like ‎Sex, age, ‎and A1C were ‎recorded too. Amplicon size was 454 bp. The specificity and ‎sensitivity ‎of the PCR reaction were100% and 50 ‎Copy/reaction respectively. The ‎analysis results showed that neither ‎the genome of HSV-1 nor 2 was found in those ‎‎100 serum samples. ‎ ‎In ‎addition, there was no relation between sex or A1C level ‎and HSV genome ‎‎presence. But it seems the relatively young age of this group is ‎effective for obtaining ‎‎these negative results. The small size of this population with ‎negative PCR ‎‎results, clearly shows that HSV infection cannot be a first-order ‎risk ‎factor, but due to time-consuming ‎mechanisms ‎of probable effects, this may affect ‎elderly populations, as obtained results of some studies. ‎Therefore to prevent or ‎manage type 2 diabetes, still should be more ‎focused on the ‎conventional risk ‎factors such as obesity and malnutrition.‎

Keywords

• Herpes Simplex Viruses
• diabetes
• PCR
• infectious diseases
• metabolic disorder‎s

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