Investigation of Frequency, Etiology, and Pattern of Antibiotic Resistance in Exogenous and Endogenous Bacterial Endophthalmitis Patients Referred to Khatam-Al-Anbia Hospital in Mashhad in 2023

Document Type : Research Article

Authors

• Mahboobeh Nakhaei Moghaddam ¹
• Arman Hasanzadeh ²
• Mohammad Reza Shayegan ³

¹ Department of Biology, Faculty of Sciences, Islamic Azad University, Mashhad Branch, Mashhad, Iran

² Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

³ Department of Medical sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran

10.22034/ijmcm.2024.710068

Abstract

Endophthalmitis is an eye infection that can lead to blindness if untreated. This study aimed to assess bacterial endophthalmitis in patients. It focused on sex differences and antibiotic resistance. It took place in 2023 at Khatam-Al-Anbia Hospital in Mashhad. It involved 60 patients aged 7 to 60. Researchers used biochemical and microbiological methods to identify bacteria. They followed CLSI M100-2023 guidelines to test antibiotic resistance. PCR was also performed to confirm the identification of the isolates. Of 60 samples suspected of ocular endophthalmitis, 15 were infected with bacteria. This included 5 isolates of Escherichia coli, 2 isolates of Pseudomonas aeruginosa, 4 isolates of Staphylococcus epidermidis and 4 isolates of Staphylococcus aureus .Gram-positive and Gram-negative bacteria showed the highest resistance to the Clindamycin (70%) and Imipenem (33%), respectively. The results of culture and biochemical tests were the same as those of PCR. Due to unsupervised use, antibiotic resistance is rising. So, we must identify resistance patterns for timely treatment.

Keywords

• Endophthalmitis
• Antibiotic Resistance
• Exogenous
• Endogenous
• Bacteria

Main Subjects

• Clinical Microbiology

---