Document Type : Research Article
Authors
1
Dep. Microbiology, Facuity of Biological science, Islamic Azad University,Tehran north Branch,Tehran,Iran
2
M. Sc of Microbial Biotechnology, Faculty of Biological science, Department of Biotechnology, Tehran North Branch, Islamic Azad University, Tehran, Iran
3
. Associate Professor of cellular and molecular Biology, Faculty of Biological science, Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahr-e-Rey Branch, Islamic Azad University, Tehran, Iran
10.22034/ijmcm.2024.710128
Abstract
Ganoderma lucidum has effective anti-cancer compounds. Chitosan has the capability of encapsulating and stabilizing Ganoderma compounds, in addition to its antioxidant properties. Separation of Ganoderma lucidum polysaccharides was carried out by hot-water extraction and treatment with 96% ethanol and protein dehydration. Using the ion exchange copolymerization method, the Ganoderma’s polysaccharides were stabilized on the chitosan nanoparticle. The morphology and type of nanoparticle were determined by SEM and FTIR methods. After the treatment of MCF-7 cells with different concentrations of nanoparticles, as well as polysaccharide extracted from the fungus, expression of the two HER3 and p53 genes was determined using the method Real-time PCR. The phenol sulfuric acid test showed the presence of 23.77 μg/ml polysaccharide in the Ganoderma lucidum. Also, Real-time PCR showed that chitosan-polysaccharide nanoparticle increased the gene expression of p53 by 7.3-fold while the expression of the HER3 gene decreased by 0.36-fold change. Also, the polysaccharide grew the expression of the p53 gene by 2.5-fold change and the HER3 gene expression fell by 0.48-fold change. The combination of chitosan and Ganoderma polysaccharides nanoparticle affects the expression of p53 and HER3 genes, causing resistance to tumor cell.
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