Contamination of quail flocks with Ornithobacterium rhinotracheale in West Azarbaijan province of Iran

Document Type : Research Article

Authors

• Ahmad Bastani ¹
• mehdi rezaei ²
• Mohammadreza asgharzadeh ³
• Sajjad Abedini Chamgordani ¹

¹ D.V.M Graduated, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran.

² Department of Clinical Sciences, Faculty of Veterinary Medicine, Ur.C., Islamic Azad University, Urmia, Iran

³ Assistant Professor, Department of Biology, Faculty of Basic Science, Urmia Branch, Ur.C., Islamic Azad University, Urmia, Iran.

10.22034/ijmcm.2025.710278

Abstract

Abstract
Background: Ornithobacterium rhinotracheale (ORT) is one of the bacterial respiratory diseases in birds, which together with other pathogenic factors, plays an essential role in reducing the performance of breeding flocks and causes heavy economic losses to the poultry industry. The aim of this study was the isolation the bacteria by microbial culture and molecular detection of ORT by polymerase chain reaction (PCR). 130 tracheal swabs were collected from 13 quail flocks that were kept in cages. After extraction of DNA from swab materials, the band of 784 bp based on the 16S rRNA gene was investigated using specific primers. The bacteria were isolated from tracheal swabs of 3 herds (23.07%) by culture method. Following DNA extraction from trachea samples, the presence of bacteria was confirmed in 4 of 13 flocks (30.76%) by PCR assay. The results of this study, for the first time, demonstrated the presence of infection in quail flocks in the West Azarbaijan province of Iran. The PCR was a suitable method for the diagnosis of ORT. Due to the proof of the presence of ORT infection, vaccination is necessary for quail breeding farms in the West Azarbaijan province of Iran.

Keywords

• Iran
• DNA
• PCR
• gene 16sR rRNA
• Poultry

Main Subjects

• Molecular Epidemiology

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