Document Type : Research Article
Authors
1
Department of Orthodontics, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran
2
Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
3
Department of Microbiology and Virology, Zanjan University of Medical Sciences, Zanjan, Iran
4
Department of Pharmaceutical Biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
10.22034/ijmcm.2024.710025
Abstract
Considering the novelty of nanotechnology and its applications in dentistry, and the existing conflicts and scarcity of studies regarding the antimicrobial activity of TiO2, ZnO, and SiO2 nanoparticles, this study aimed to fabricate acrylic nanocomposites containing different percentages of mineral nanoparticles and assess their antimicrobial activity.
In this in vitro, experimental study, standard-strain Streptococcus mutans (S. mutans) (ATCC35668) and Candida albicans (C. albicans) (ATCC10231) were cultured and biochemically confirmed. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of zinc oxide (ZnO), titanium dioxide (TiO2), silver (Ag), and silicon dioxide (SiO2) nanoparticles were determined by the broth macrodilution and agar well diffusion method. Remarkably, the results revealed that, TiO2, SiO2, and ZnO in 1, 0.5, 0.25, 0.125, and 0.062wt% concentrations had no inhibitory effect against S. mutans and C. albicans. While, silver nanoparticles (AgNPs) showed in vitro activity against S. mutans (MIC=MBC 62.5 µg/ml) and C. albicans (MIC 125 µg/ml) . However, nanocomposites containing 0.125, 0.25, 0.5, 1, and 2wt% TiO2 had no in vitro activity against S. mutans and C. albicans. In the present study, only AgNPs showed antimicrobial activity against S. mutans and C. albicans in 1, 0.5, 0.25, 0.125, and 0.062wt% concentrations. However, further studies are warranted to correlate these findings with clinical outcomes.
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